Phytohemagglutinin
The entire physical, biochemical and physiological makeup of an individual as determined both genetically and environmentally.
A person formally trained to provide diagnostic, therapeutic, and preventive health care services with physician supervision. A type of Mid-Level Practitioner.
A plant lectin used to polyclonally stimulate T lymphocytes.
Platelet
Capable of producing cells of different lineages.
The first practical system for in vitro amplification of DNA, and as such one of the most important recent developments in molecular biology. Two synthetic oligonucleotide primers, which are complementary to two regions of the target DNA (one for each strand) to be amplified, are added to the target DNA (that need not be pure), in the presence of excess deoxynucleotides and Taq polymerase, a heat-stable DNA polymerase. In a series (typically 30) of temperature cycles, the target DNA is repeatedly denatured (around 90°C), annealed to the primers (typically at 50-60°C) and a daughter strand extended from the primers (72°C). As the daughter strands themselves act as templates for subsequent cycles, DNA fragments matching both primers are amplified exponentially, rather than linearly. The original DNA need thus be neither pure nor abundant, and the PCR reaction has accordingly become widely used not only in research, but also in clinical diagnostics and forensic science.
Enzymes involved in template-directed synthesis of DNA from deoxyribonucleotide triphosphates. I, II and III are known in E. coli ; III appears to be most important in genome replication and I is important for its ability to edit out unpaired bases at the end of growing strands. Animal cells have alpha, beta or gamma polymerases, with alpha apparently responsible for replication of nuclear DNA, and gamma for replication of mitochondrial DNA. All these function with a DNA strand as template. Retroviruses possess a unique DNA polymerase (reverse transcriptase) that uses an RNA template.
Variant forms of a particular gene that occurs simultaneously in a population.
The manipulation of a cellular therapy product such that a specific cell population(s) is enriched.
The measure of the degree of repeatability of a method under normal operation. Normally expressed as the percent relative standard deviation for a statistically significant number of samples.
Early ancestor of a given cell.
A sequence of DNA or RNA, labeled or marked with a radioactive isotope, used to detect the presence of complementary nucleotide sequences.
Documents that explain how the goals of the laboratory will be achieved and serve as guidelines that define the general course and scope of activities permissible for goal accomplishment. Describes the goal-directed interrelated series of actions, events, or steps that are required.
Includes all aspects of manipulation, labeling, and infusion of products, regardless of source.
The harvested material that is being processed at any stage of the processing. Formally referred to as component.
An evaluation of the ability to perform laboratory procedures within acceptable limits of accuracy, through and analysis of unknown specimens distributed at periodic intervals by a source outside the facility performing the proficiency test.
Cell precursor.
The production of new cells from a given precursor.
Describing the removal of one or more cell populations from a cell suspension.
