Fluorescent Activated Cell Sorter

Foundation for the Accreditation of Cellular Therapies

Fetal Bovine Serum

Food and Drug Administration

Reagent applied for density gradients.

Fluorescein isothiocyanate

A machine that measures cell size, granularity and fluorescence due to bound florescent antibodies as single cells pass in a stream through a laser light and photo detectors.  The analysis of single cells in this way is called flow cytometry and the instruments that carry out the measurements are called flow cytometers.

An unexpanded cellular therapy product that has never been cryopreserved.  May also refer to products that are ex vivo expanded from previously frozen cells.

Granulocyte colony stimulating factor

The insertion of one or more exogenous genes into hematopoietic cells or other cell types.

Granulocyte-macrophage colony stimulating factor

Manifestations of the reaction of engrafted donor cells against host tissue; clinical symptoms involve the skin, liver, and intestinal tract with potential for effect on additional organs.  Acute GVHD occurs usually within the first 100 days post transplant and is initiated by T lymphocytes of donor origin.  Chronic GVHD is generally seen after the first 100 days and may also be caused by donor T cells, but cytokines (IFN-g, TNF, IL-1) may also play a role in the disease process.

The immune mediated elimination of residual leukemia by donor-derived cells infused with the stem cell graft.  Similar effects in diseases other than leukemia are termed Graft-versus-Tumor reactions.

Any cell containing granules; leukocyte containing neutrophil, basophil or eosinophil granules in its cytoplasm.

A hematopoietic growth factor that induces the growth and differentiation of progenitor cells committed to the neutrophil lineages and enhances the functional activities of effector cells of the granulocyte lineage.  Used clinically to increase the rate of granulocyte recovery following chemotherapy and transplantation, and to induce CD34⁺ hematopoietic progenitor cells into the circulation for the collection of peripheral blood stem cells.

A hematopoietic growth factor that regulates the growth and differentiation of progenitor cells and enhances the functional activities of effector cells of the granulocyte-macrophage lineage.  Used clinically to increase the rate of granulocyte recovery following chemotherapy and transplantation, and to induce CD34⁺ hematopoietic progenitor cells into the circulation for the collection of peripheral blood stem cells.

Good Tissue Practices

Graft versus host disease

Graft versus leukemia

The collection of HPC-M, HPC-A or other products for use in transplantation.